Ngwaahịa
HEPES sodium nnu
Ụdị okwu: HEPES sodium nnu; 75277-39-3; HEPES hemisodium nnu; 103404-87-1; sodium 2- (4- (2-hydroxyethyl) piperazin-1-yl) ethanesulfonate; sodium 2-[4- (2-hydroxyethyl). Piperazin-1-yl] etanesulfonate; HEPES (sodium); C8H17N2NaO4S; 4- (2-Hydroxyethyl) piperazine-1-ethanesulfonic acid hemisodium nnu; 1-Piperazineethanesulfonic acid, 4- (2-hydroxyethyl) -, monosodium nnu; 4- (2-Hydroxyethyl) piperazine-1- ethanesulfonic acid sodium nnu; 4- (2-hydroxyethyl) piperazin-1-ylethanesulphonate, UNII-Z9FTO91O8A; Z9FTO91O8A; sodium; 2-[4- (2-hydroxyethyl) piperazin-1-yl] ethanesulfonate; DTXSID4044458; CHEBI: 4675 278-169-7; 1-Piperazineethanesulfonic acid, 4- (2-hydroxyethyl) -, sodium nnu (1: 1); N- (2-Hydroxyethyl) piperazine-N'-2-ethanesulfonic acid, sodium nnu; HEPES sodium nnu,> = 99.5% (titration); sodium hepes; N- (2-Hydroxyethyl) piperazine-N'- (2-ethanesulfonic acid) sodium nnu; MFCD00036463; HEPES, sodium nnu; HEPES sodium nnu, 98%;C8H18N2O4S.Na;SCHEMBL229142;WAS-14;CHEMBL3187284;DTXCID2024458;RDZTWEVXRGYCFV-UHFFFAOYSA-M;C8-H18-N2-O4-SNa ngwọta nnu sodium; (1M); Tox21_302155; HB5187; N- (2-Hydroxyethyl) piperazine-N- (2-ethanesulfonicacid) hemisodiumsalt; HEPES sodium nnu, biochemical. ọkwa;AKOS015897769;AKOS015912229;AKOS015964204;NCGC00255791-01;CAS-75277-39-3;HY-108535;CS-0029103;FT-06108Asodium; N'-hydroxyethyl-N-piperazineethanesulfonate; HEPES sodium nnu, BioXtra,> = 99.0% (titration); HEPES sodium nnu, Vetec (TM) reagent ọkwa, 96%; W-104397; Q27120698; sodium2- (4- (2-hydroxyethyl) piperazin-1-yl) ethanesulfonate; 1-piperazineethanesulfonic acid, monosodium nnu; N- (2-Hydroxyethyl) piperazine-N'- (2-ethanesulfonic acid) sodiumsalt; N-2-Hydroxyethylpiperazine-N'-2-ethanesulphonic acid hemisodium nnu, HEPES sodium nnu, BioPerformance akwụkwọ, dabara maka omenala cell, >> 99.5%
Ngwongwo kemịkalụ nke HEPES Sodium nnu
● Ọdịdị / Agba: ọcha ntụ ntụ
● Uzuoku ike: 0Pa na 25 ℃
● Ebe Na-agbaze: 234 Celsius
● PKA: 7.5 (na 25 ℃)
● PSA:92.29000
● Njupụta:1.504[na 20℃]
● LogP: -0.90190
● Okpomọkụ Nchekwa: Ụlọ ahịa na RT.
● Mmetụta.: Hygroscopic
● Solubility.: H2O: 1 M na 20 Celsius C, doro anya, enweghị agba
● Mmiri Solubility.: Ọ na-agbaze na mmiri.
● Ọnụọgụ ndị na-enye onyinye nke hydrogen:1
● Ọnụọgụ nnabata nke njikọ hydrogen:6
● Ọnụọgụ nke nkwekọrịta na-agbanwe agbanwe: 5
● Kpọmkwem Mass:260.08067248
● Ọnụ ọgụgụ atọm dị arọ:16
● Mgbagwoju anya:272
Ozi nchekwa
● Foto:
● Koodu ihe egwu:
● Okwu Nchekwa: 22-24/25
Bara uru
Klas kemịkalụ:Ojiji ndị ọzọ -> Ihe nchekwa ndu
Ọchị ọchị nke Canonical:C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+]
Na-eji:HEPES sodium nnu ngwọta dabara adaba maka ọmụmụ omenala cell. Enwere ike iji ya na nkwadebe nke ngwọta nchekwa nke HEPES. Enwere ike iji ya dị ka ihe nchekwa n'oge nyocha nke phosphorylation na fibroblasts permeabilized. Enwere ike iji ya mepụta ihe nchekwa dialysis dị mgbagwoju anya, nke ejiri mee mkpebi nke thyroxin efu na serum site na radioimmunoassay. HEPES sodium nnu ngwọta dabara adaba maka ojiji na ọmụmụ omenala cell. Enwere ike iji ya na nkwadebe nke ngwọta nchekwa nke HEPES. Enwere ike iji ya dị ka ihe nchekwa n'oge nyocha nke phosphorylation na fibroblasts permeabilized. Enwere ike iji ya mepụta ihe nchekwa dialysis dị mgbagwoju anya, nke ejiri mee mkpebi nke thyroxin efu na serum site na radioimmunoassay.
Okwu Mmalite zuru ezu
HEPES sodium nnu, nke a makwaara dị ka 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid sodium nnu, bụ ụdị HEPES a na-ejikarị eme ihe. Ọ bụ ngwakọta organic zwitterionic nke na-arụ ọrụ dị ka onye na-echekwa ihe na nyocha ihe ndụ na biochemical.
A na-ejikarị nnu sodium HEPES eme ihe na mgbasa ozi omenala cell na ihe nchekwa ndụ n'ihi ikike ya idowe pH kwụsiri ike gburugburu ọnọdụ physiological (pH 7.2 - 7.6). Ọ bụ nke ukwuu soluble na mmiri na nwere dịtụ ala toxicity, na-eme ka ọ dabara maka dị iche iche ngwa.
A na-ahọrọ ụdị nnu sodium nke HEPES n'ọtụtụ ngwa ka ọ na-eme ka solubility na nkwụsi ike, ma e jiri ya tụnyere ụdị acid free. Ọ na-ere ahịa dị ka ntụ ntụ kristal ọcha nke nwere ike ịgbaze ngwa ngwa n'ime mmiri iji kwadebe ngwọta na-arụ ọrụ.
Ndị na-eme nchọpụta na-ejikarị nnu sodium HEPES eme ihe na usoro ihe ọmụmụ ihe ndị dị ndụ, omenala cell, nyocha enzyme, ịdị ọcha protein, na nyocha biochemical ndị ọzọ ebe njikwa pH dị mkpa. Ikike nchekwa ya na ndakọrịta na sistemu ndu na-eme ka ọ bụrụ nhọrọ a ma ama maka idowe ọnọdụ kacha mma maka ọrụ na nkwụsi ike nke ụmụ irighiri ihe dị ndụ.
Tupu iji nnu sodium HEPES n'ime ngwa ọ bụla akọwapụtara, ọ dị mkpa iji nyochaa akwụkwọ ndị ahụ, lelee ndụmọdụ ndị na-ebubata ya, wee kwalite ntinye uche na pH dabere na ihe nnwale achọrọ.
Ngwa
HEPES sodium nnu na-achọta ngwa zuru oke na mpaghara nyocha dị iche iche nke ndu na biochemical. Ụfọdụ n'ime isi ihe eji eme ya gụnyere:
Omenala cell:A na-agbakwunye nnu sodium HEPES na mgbasa ozi omenala cell iji nọgide na-enwe pH kwụsiri ike n'ime usoro physiological ma nye ebe kwesịrị ekwesị maka mkpụrụ ndụ na-eto eto na ịgbasa.
Ihe nnọchi anya:A na-arụ ọrụ nnu sodium HEPES ugboro ugboro dị ka onye na-echekwa ihe na ihe nchekwa ndu yana ngwọta, gụnyere nyocha enzyme, ịdị ọcha protein, na usoro ihe ọmụmụ ihe ndụ. Ọ na-enyere aka ịnọgide na-enwe pH mgbe niile site na iguzogide mgbanwe na acidity ma ọ bụ alkalinity.
Electrophoresis: A na-eji nnu sodium HEPES mee ihe dị ka ihe nchekwa na gel electrophoresis iji nọgide na-enwe pH kwụsiri ike n'oge nkewa nke acid nucleic ma ọ bụ protein. Ọ na-egbochi mgbanwe na pH nke nwere ike imetụta njem na nkewa nke ụmụ irighiri ihe dị na gel.
Protein kwụsie ike: A na-agbakwunye nnu sodium HEPES na ngwọta protein iji mee ka nhazi ha kwụsie ike ma nọgide na-arụ ọrụ ha. Ọ na-enyere aka ịnọgide na-enwe ezigbo pH gburugburu dị mkpa maka nkwụsi ike protein na ịrụ ọrụ.
Enzyme ọrụ: A na-eji nnu sodium HEPES mee ihe dị ka ihe nchekwa na mmeghachi omume enzymatic iji dowe pH achọrọ maka ọrụ enzyme kacha mma. Ọ na-enyere aka hụ na enzymes na-arụ ọrụ na arụmọrụ kachasị elu na nke ziri ezi.
Onyonyo ekwentị dị ndụ:HEPES sodium nnu na-etinyekarị na mgbasa ozi onyonyo maka nnwale onyonyo cell ndụ. Ikike nchekwa ya na-enyere aka idowe pH a chọrọ ma gbochie mgbanwe ndị nwere ike imetụta viability na fluorescence nke sel.
Usoro ihe omimi ihe omimi: A na-eji nnu sodium HEPES rụọ ọrụ n'ọtụtụ ụzọ na usoro ihe ọmụmụ ihe dị iche iche, gụnyere DNA ma ọ bụ RNA iche, PCR, usoro DNA, na nyocha protein. Ikike nchekwa ya na-enye ohere maka nsonaazụ ziri ezi na ntụkwasị obi n'oge usoro ndị a.
Ọ dị mkpa iburu n'obi na ngwa na itinye uche nke nnu sodium HEPES nwere ike ịdịgasị iche dabere n'ihe nyocha chọrọ yana usoro ndu nke a na-amụ. Ya mere, ọ bụ ihe amamihe dị na ya ịkpọtụrụ akwụkwọ na ndụmọdụ ndị na-ebubata ngwaahịa maka iji ezigbo ya.
